The PLEX-ID Pneumococcus Serotyping assay is a qualitative, non-diagnostic assay intended for the detection, identification, and characterization of Streptococcus pneumoniae from culture isolates, through nucleic acid amplification and subsequent mass spectrometry analysis.
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID Pneumococcus Serotyping assay kit includes:
- 10 Plates (120 assays)
- Plate Processing Protocol
- DNA Dilution Buffer
- PCR Enzyme Dilution Buffer
- PLEX-ID DNA Polymerase
Positive, negative, or extraction controls are not provided.
The PLEX-ID Pneumococcus Serotyping assay uses 40 primer pairs multiplexed into eight wells to detect and identify over 40 antigenically distinct serogroups representing 91 serotypes of Streptococcus pneumoniae.
Additional Information
- Hiller NL, Ahmed A, Powell E, Martin DP, Eutsey R, et al. (2010) Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection. PLoS Pathog 6.
- Jacobs MR, Bajaksouzian S, Bonomo RA, Good CE, Windau AR, et al. (2009) Occurrence, distribution, and origins of Streptococcus pneumoniae Serotype 6C, a recently recognized serotype. J Clin Microbiol 47: 64-72.
